Artifact Correction for Zernike Phase-Plate Cryo-Electron Tomography
نویسندگان
چکیده
In cryo-electron microscopy (cryo-EM), vitreously frozen biological specimens can tolerate only a limited electron dose for imaging. Therefore, cryo-EM micrographs display weak contrast and a poor signal-to-noise ratio. This limits data interpretability and prevents efficient application of cryo-EM to a wider range of biological problems. Continued efforts have been invested in imaging methods using phase plates for cryo-EM, as this technique can offer greatly enhanced image contrast [1-3]. While various types of phase-plate are under development, Zernike-type phase-plate imaging has been successfully applied to a number of cryo-EM studies in both single-particle and electron tomographic studies [4-9].
منابع مشابه
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